在感染传染病病毒感染后发生的血清代谢变化
Dan Wang1, Jiangwei Song1, Jing Wang1
1Beijing Key Laboratory for Prevention and mock of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Garden Middle Road Haidian District, Beijing, 100097, China.
BMC veterinary research
|December 19, 2024
概括
传染性病病毒 (IBDV) 感染显著改变血清代谢,特别是影响氨基酸和脂质代谢. 这些代谢变化突出了宿主.
科学领域:
- 兽医病毒学 兽医病毒学
- 代谢学 代谢学 代谢学
- 免疫抑制是一种免疫抑制.
背景情况:
- 传染病病毒 (IBDV) 是一种高度传染性的免疫抑制性病原体,在中,通常通过口服获得.
- 虽然IBDV的病理影响是已知的,但它对血清代谢物的影响以前没有报告过.
- 这项研究调查了在感染减弱 (atIBDV) 和非常毒性 (vvIBDV) 菌株后的代谢变化.
研究的目的:
- 鉴定感染IBDV.的的血清代谢概况变化.
- 为了确定特定的代谢物和受IBDV和vvIBDV感染影响的途径.
- 了解宿主对IBDV感染的代谢反应.
主要方法:
- 在SPF中,口服接种了atIBDV菌株LM或vvIBDV菌株LX.
- 血清样本是在感染后的第七天收集的.
- 超高性能液态色谱-并联质谱 (UHPLC-MS/MS) 用于代谢物识别,随后进行多变量统计分析和KEGG通路丰富分析.
主要成果:
- 在对IBDV和vvIBDV感染的反应中,共发现了368种显著变化的代谢物.
- 在氨基酸和脂质代谢中观察到代谢障碍,特别是涉及托,甘油脂,氨酸和氨酸途径.
- 具体的差异代谢物包括各种脂 (如PC,PE,PS),氨基酸衍生物和醇化合物.
结论:
- 无论毒性如何,IBDV感染都会导致血清代谢发生显著变化.
- 鉴定的代谢失调提供了对宿主对IBDV的反应机制的见解.
- 代谢分析提供了一种新的方法来了解IBDV的发病过程.
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