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单细胞RNA测序揭示了暴露于食品添加剂 (E171) 二氧化的异质免疫细胞反应

Haribalan Perumalsamy^1,2,3, Xiao Xiao³, Hyoung-Yun Han⁴

¹Institute for Next Generation Material Design, Hanyang University, Seoul, 04763, Republic of Korea.

Journal of nanobiotechnology

|December 19, 2024

概括

暴露于老鼠中的二氧化 (E171 TiO2) 揭示了复杂的免疫反应,不同的免疫细胞表现出不同的促炎或抑制作用. 这项研究强调需要对食品添加剂对免疫系统的影响进行详细评估.

科学领域:

免疫学免疫学免疫学
毒理学毒理学毒理学
纳米技术纳米技术

背景情况:

二氧化 (E171 TiO2) 是一种广泛使用的食品添加剂,有可能对免疫系统产生相互作用.
较小的TiO2颗粒可以进入血液,引发各种免疫反应.
在异质免疫细胞群中,E171 TiO2的特定免疫和转录效应尚不清楚.

研究的目的:

为了研究食品添加剂E171 TiO2暴露的细胞和免疫反应.
为了阐明E171 TiO2管理后个别免疫细胞类型的转录组形状.
评估E171 TiO2对先天性和适应性免疫反应的影响.

主要方法:

雄性Sprague-Dawley大鼠通过内灌注暴露于E171 TiO2 (5 mg/kg bw/day) 在13周内.
在采集血液样本之前,实施了为期4周的恢复期.
单细胞RNA测序 (scRNAseq) 在血液样本上进行,以分析免疫细胞反应.

主要成果:

颗粒细胞G1细胞表现出激活的先天性免疫反应,升高调节促炎性细胞毒性基因.
自然杀手 (NK) 细胞子集表现出异质的反应:NK1抑制了细胞毒性,而NK2和NK3调节了B和T细胞活性.
与MHC-I/II复合体相互作用的T细胞子集没有显示功能障碍或抑制细胞毒性T细胞,这表明适应性免疫不被抑制.

结论:

E171 TiO2暴露会在单细胞水平上诱导各种免疫和转录基因反应.
不同的免疫细胞子集,包括NK和NKT细胞,在对TiO2的反应中表现出功能异质性.
该研究提供了一种高维的方法,以精确评估食品添加剂的免疫影响,如E171TiO2.

关键词:

E171 TiO2 含有 E171 的二氧化.异质性异质性异质性免疫反应的免疫反应.鼠外周血液中的单核细胞.在 scRNAseq 分析中,

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