揭示了新的和保守的CD8+T细胞表位与ALV-J上的MHC B2限制
Xueqing Li1, Ziwei Li1, Mulin Ma1
1National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
研究人员在感染禽类白血病病毒亚组J (ALV-J) 的中确定了三个关键的CD8+T细胞表位. 这些由MHC B2限制的表位对开发有效的T细胞疫苗对抗ALV-J和相关病毒至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 遗传学 遗传学 是一个
背景情况:
- 在MHC B2型中,具有强烈的免疫反应.
- 关于受MHC B2限制的CD8+T细胞表观体对禽类白血病病毒亚组J (ALV-J) 的知识有限.
研究的目的:
- 为了探索ALV-J诱导的细胞免疫反应在B2单元型.
- 在ALV-J.上识别MHC B2受限的CD8+T细胞表位.
主要方法:
- 在活体ALV-J感染模型在B2单元型中.
- 对CD8+T细胞比例和基因表达的分析 (Granzyme A,IFIT5).
- 基于结合基因和ELISpot测定IFN-γ,TNF-α和IL-2生产的类选择.
主要成果:
- ALV-J 感染增加了 CD8+ T 细胞和上调的细胞毒性/抗病毒基因.
- 鉴定了三种MHC B2受限的CD8+T细胞表位 (Pol652-660,Gag374-382,Gag403-411),其中包括三种MHC B2受限制的CD8+T细胞表位.
- 这些表位突变诱导了显著的IFN-γ,TNF-α和IL-2产生,并且在多个ALV亚组中得到保存.
结论:
- 在ALV-J.上鉴定了三种新的MHC B2受限制的CD8+T细胞表位.
- 这些表位细胞为开发针对性T细胞表位细胞疫苗开发针对保存ALV蛋白的基础.
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