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整合性蛋白质学和光蛋白质学分析症状辅助骨的概况,基于并联式质量标记技术

Bin Liu¹, Ran Wei¹, Yuqing Wang¹

¹Department of Orthopaedics, Bethune International Peace Hospital, Shijiazhuang, 050082, China.

International journal of general medicine

|December 19, 2024

概括

运动训练后的辅助 navicular 骨 (ANB) 损伤涉及影响炎症,骨修复和微观结构的蛋白质. 这项研究揭示了与ANB相关的脚损伤的分子洞察力,以制定更好的治疗策略.

科学领域:

分子生物学分子生物学
整形外科整形外科整形外科
蛋白质组学是指蛋白质组学.

背景情况:

辅助 navicular 骨 (ANB) 可以损害足部功能,并在运动训练后导致受伤.
运动训练后与ANB相关的足部损伤背后的分子机制尚不清楚.

研究的目的:

研究运动训练后ANB患者脚损伤的蛋白质组和蛋白质组概况.
确定差异表达蛋白 (DEP) 和酸化蛋白 (DPP),并阐明它们在ANB相关的脚损伤中的作用.

主要方法:

使用双重质量标记 (TMT) 技术对5名患者的ANB和正常骨 (NB) 组织进行蛋白质组和光蛋白质组测序.
对DEP和DPP的识别和功能注释.
为DEP建立一个蛋白质与蛋白质相互作用 (PPI) 网络.

主要成果:

确定了147个DEP (129个上调,18个下调),富含细胞外基质 (ECM) 受体相互作用和细胞粘附通路.
在PPI网络中,COL4A1,COL4A2,RBBP4和RBBP7的互动得分很高.
确定了4个上调和1个下调的DPP,主要参与调节脂肪细胞中的脂解.

结论:

运动训练后ANB带来的脚损伤与参与炎症,免疫,骨修复,再生和微观结构稳定性相关的蛋白质有关.
已识别的蛋白质 (例如,MRC1,COL4A1,COL4A2,ITGA9) 提供了关于ANB相关脚损伤病原学的新见解.
这些发现可能有助于制定针对ANB相关脚损伤的向治疗策略.

关键词:

配件船骨的船骨.运动员训练运动员训练光蛋白质组学光蛋白质组学光蛋白质组学蛋白质组学蛋白质组学

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