相关实验视频

Updated: Jun 4, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

使用LC-QTOF-MS的单/多表位亲缘关系技术对Trastuzumab修饰分析进行比较研究

Chengyi Zuo¹, Jingwei Zhou¹, Sumin Bian²

¹Institute of Pharmaceutical Analysis, College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research of China, Jinan University, Guangzhou, 510632, China.

Journal of pharmaceutical analysis

|December 19, 2024

概括

一种新的多表位相亲技术增强了在体内单克隆抗体 (mAb) 生物转换的跟踪. 这种新的平台准确地监测生物流体中的关键mAb修饰,改进药物疗效分析.

科学领域:

生物化学生物化学
分析化学分析化学
材料科学材料科学材料科学

背景情况:

活体中单克隆抗体 (mAb) 生物转化的动态跟踪对于药物有效性至关重要.
抗原结合部位的修改可能导致免疫识别问题,复杂化分析.
现有的亲和材料在检测关键地点修改方面存在局限性.

研究的目的:

为增强体内mAb生物转化分析开发一种新型的多表位相亲技术.
克服传统方法在检测关键的mAb修改方面的局限性.
建立一个敏感的生物分析平台,用于跟踪生物流体中的trastuzumab修饰.

主要方法:

一种复合材料的制造:金属有机框架 (MOF) @Au@peptide@aptamer.
同时固定互补性确定区域 (CDR) 仿原蛋白 (HH24) 和非CDR仿原蛋白 (CH1S-6T).
整合液体染色学-飞行质谱学四极时间 (LC-QTOF-MS) 进行分析.

主要成果:

在MOF@Au@peptide@aptamer复合物表现出优越的丰富的trastuzumab变体相比,单位的方法.
该平台成功地监测了特定部位的脱化和异构化 (LC-Asn-30,HC-Asn-55).
与缓冲剂相比,在血清中观察到更快的修饰趋势,归因于生物学因素.

结论:

多表位亲缘关系技术有效地解决了 mAb 修饰分析传统方法中的偏差.
这种新型生物分析平台能够在体内准确跟踪各种生物流体中的mAb变化.
这种方法在推进mAb研究和临床应用方面具有重大潜力.

关键词:

生物转化分析分析乳腺癌乳腺癌乳腺癌这就是LC-QTOF-MS.单克隆抗体是一种单克隆抗体.多层表皮相亲技术多层表皮相亲技术.

更多相关视频

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Published on: September 14, 2018

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Published on: June 16, 2023

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...