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Updated: Jun 14, 2026

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吉拉德基于衍生化的丰富策略,用于对生物样本中的碳基基代谢组进行分析

Xin Tao1, Jia-Yue Liu1, Jun-Yi Zhou1

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR 999078, China.

Analytical chemistry
|December 19, 2024
PubMed
概括

这项研究引入了一种新方法来检测碳基代谢物,这些代谢物是疾病的重要生物标志物. 基拉德衍生化基础丰富 (GDBE) 策略提高了检测灵敏度,并在小鼠肝脏组织中识别了许多代谢物.

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相关概念视频

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...