由生物标志物驱动的逻辑门激活的CRISPR/RNA Aptamer系统
Qiqi Yang1,2, Ming-Jie Dong3, Jianglian Xu1
1Marshall Laboratory of Biomedical Engineering, Precision Medicine and Health Research Institute, Shenzhen Key Laboratory for Nano-Biosensing Technology, Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518060, China.
Journal of the American Chemical Society
|December 19, 2024
概括
我们开发了InCasApt, 一种用于检测癌症生物标志物和提供向治疗的RNA装置. 这种纳米技术平台使用CRISPR Cas13a和aptamer来激活瘤细胞的治疗,
科学领域:
- 生物技术
- 纳米技术
- 分子生物学
背景情况:
- 工程RNA装置对于检测生物标志物和启用响应性疗法至关重要.
- 目前的诊断平台需要先进的综合诊断和治疗.
- 针对特定的microRNAs (miRNAs) 在癌症治疗中具有前景.
研究的目的:
- 开发一个集成的纳米CRISPR Cas13a/RNA 体测试平台 (InCasApt).
- 实现同时检测生物标志物和生物标志物驱动的治疗.
- 研究InCasApt在瘤细胞中向致癌miRNA的疗效.
主要方法:
- 染色Cas13a/crRNA复合物,头针记者,Ce6光敏感剂和胺前体到树突性半孔纳米粒子 (DMSN).
- 通过瘤细胞中miRNA-155和miRNA-21表达升高而激活的AND逻辑门机制.
- 使用光用于疾病评估和反应性氧物种 (ROS) 产生用于光动力学治疗.
主要成果:
- InCasApt在正常细胞中表现为惰性,在高miRNA-155和miRNA-21水平的瘤细胞中表现为活化.
- 该平台成功地产生了用于疾病检测的光和用于光动力疗法的ROS.
- InCasApt通过抑制标miRNAs来上调抗原基因BRG1并抑制瘤迁移.
结论:
- InCasApt是用于综合癌症诊断和治疗的多功能miRNA向策略.
- 该平台有效地弥合了疾病检测和治疗干预之间的差距.
- 这种工程RNA装置为瘤学中的治疗应用提供了一种新的方法.
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