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Insulin: The Receptor and Signaling Pathways01:28

Insulin: The Receptor and Signaling Pathways

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Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
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在体外诱导胰岛素耐药性

Eng Leng Saw1

  • 1Whitaker Cardiovascular Institute, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA. elsaw@bu.edu.

Methods in molecular biology (Clifton, N.J.)
|December 19, 2024
PubMed
概括

这项研究详细介绍了一种使用棕酸盐和葡萄糖诱导人类心脏细胞抗胰岛素的方法. 这种细胞模型有助于研究2型糖尿病和肥胖等慢性代谢障碍.

科学领域:

  • 生物化学 生物化学
  • 细胞生物学 细胞生物学
  • 代谢研究研究 代谢研究

背景情况:

  • 细胞模型是了解疾病机制的重要临床前工具.
  • 棕酸是一种和脂肪酸,通常用于诱导体外胰岛素抵抗,用于研究代谢障碍.
  • 胰岛素抵抗是2型糖尿病和肥胖症的标志.

研究的目的:

  • 概述一种在AC16人类心脏衍生细胞中诱导胰岛素抵抗的程序.
  • 为研究胰岛素抵抗对心脏细胞的影响提供可靠的体外模型.

主要方法:

  • 使用AC16人类心脏衍生细胞.
  • 使用高度的棕酸盐和葡萄糖来诱导细胞胰岛素抵抗.
  • 专注于AKT/GLUT-4信号通路的调制.

主要成果:

  • 在AC16细胞中通过棕酸盐和葡萄糖治疗成功诱导胰岛素抵抗.
  • 证明了对AKT/GLUT-4信号通路的目标影响.
  • 建立了一个功能性细胞模型用于代谢疾病研究.

结论:

  • 描述的方法有效地在人类心脏细胞中创建了体外胰岛素耐药性的模型.
关键词:
AC16 人类心脏衍生细胞在 AKT AKT 里面.在GLUT-4中使用.葡萄糖是一种葡萄糖.胰岛素耐药性 胰岛素耐药性是一种棕酸盐是一种棕酸盐.

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Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance
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Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)

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Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance
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Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance

Published on: February 17, 2023

  • 这种模型适合研究慢性代谢障碍的机制方面.
  • 在这种情况下,AKT/GLUT-4通路是诱导和研究胰岛素耐药性的关键目标.