相关实验视频
Updated: Jun 4, 2025
08:39
Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
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定量实时PCR用于循环RNA检测和分析.
Amit Kumar Rai1, Venkata Naga Srikanth Garikipati2
1Aging + Cardiovascular Discovery Center, Department of Cardiovascular Sciences, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Methods in molecular biology (Clifton, N.J.)
|December 19, 2024
概括
循环RNAs (circRNAs) 是关键的调节性非编码RNAs. 研究人员使用实时定量PCR与分离的原料来研究circRNA表达和基因调节中的功能.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 虽然只有2%的人类基因组编码蛋白质,但超过75%是非编码的,并发挥着重要的调节作用.
- 非编码RNAs,包括微RNAs (miRNAs),小核RNAs (snRNAs),小核RNAs (snoRNAs),转移RNAs (tRNAs) 和循环RNAs (circRNAs),对于基因调节至关重要.
- 循环RNAs (circRNAs) 是一种具有显著基因调节功能的新型调节性非编码RNAs类.
研究的目的:
- 突出非编码RNAs,特别是circRNAs在真核生物基因调节中的重要性.
- 解释circRNAs发挥其调节作用的机制,例如作为miRNA或RNA结合蛋白海绵.
- 引入实时定量PCR方法,使用分离的原料进行circRNA表达分析.
主要方法:
- 定量实时PCR (qRT-PCR) 用于检测和量化circRNA表达.
- 不同原始物在qRT-PCR中专门用于准确识别和测量圆形RNA分子.
- 这种方法允许精确评估生物样本中的circRNA丰度.
主要成果:
- 循环RNAs在复杂的基因调控网络中充当强大的调节器.
- 它们可以有效地隔离miRNA和RNA结合蛋白,从而调节基因表达.
- 使用分歧原料的qRT-PCR的应用使 circRNA表达水平的可靠测量成为可能.
结论:
- 循环RNA是真核细胞调节格局的组成部分.
- 它们作为分子海绵的作用能力强调了它们在基因调节中的功能意义.
- 使用分离原料的定量实时PCR是研究circRNA表达模式的验证方法.
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