膜巨细胞是疹病毒的早期目标
Aum R Patel1,2, Amit Garg1, Haylen T Rosberger1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
概括
病毒 (MuV) 主要感染人类和小鼠模型中的单细胞和膜巨细胞 (AMs). 这确定了AMs作为早期MuV感染和在体内传播的关键目标.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 病毒 (MuV) 是一个全球性病原体,尽管疫苗接种率很高.
- 在体内Muv感染的初始细胞点仍然未确定.
- 了解早期的MuV目标对于理解其病原和传播至关重要.
研究的目的:
- 在体内识别型病毒 (MuV) 感染的早期细胞标.
- 为了研究单细胞和巨细胞对MuV感染的易感性.
- 阐明膜巨细胞 (AMs) 在MuV病变发生过程中的作用.
主要方法:
- 产生绿色光蛋白 (GFP) 标记的MuV疫苗菌株 (JL5).
- 白细胞细胞系和外围血液单核细胞 (PBMC) 感染了MuV菌株.
- 用GFP和Cre重组酶标记的MuV对Rosa26-tdTomato小鼠进行鼻腔注射.
- 用MuV感染隔离的小鼠膜巨细胞 (AMs) 和人类支气管膜洗细胞.
主要成果:
- MuV复制在单细胞中最大,这些单细胞优先感染JL5和循环的基因型G菌株.
- 单细胞衍生的巨细胞显示出高度易感性,基因型G感染它们的程度更大.
- 在小鼠的体内研究表明,膜巨细胞 (AMs) 是肺部主要的受感染细胞类型.
- 人类AMs的感染证实了它们对MuV的敏感性,大多数感染的细胞是AMs.
结论:
- 单细胞及其衍生性巨细胞是疹病毒 (MuV) 的主要细胞标.
- 膜巨细胞 (AMs) 在小鼠和人类系统中都非常容易受到MuV感染.
- 这些发现强调了AMs对于早期的MuV病原体和随后的病毒传播至关重要.
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