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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Overview
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相关实验视频

Updated: Jun 4, 2025

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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无细胞基因表达:方法和应用.

Andrew C Hunt1,2, Blake J Rasor1,2, Kosuke Seki1,2

  • 1Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.

Chemical reviews
|December 19, 2024
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概括

无细胞基因表达 (CFE) 系统使细胞外的合成生物学成为可能,在分布和生物控制方面具有优势. 最近的进步正在改变细胞结构,遗传网络控制和基于生物的产品制造.

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科学领域:

  • 合成生物学 合成生物学
  • 生物技术是生物技术.
  • 分子生物学分子生物学

背景情况:

  • 无细胞基因表达 (CFE) 系统为传统的基于细胞的生物过程提供了替代方案.
  • 这些系统在完整的细胞之外运作,利用纯化的酶或细胞溶解物进行生物分子转化.
  • CFE绕过了细胞的局限性,例如通过细胞壁传输和生存机制.

研究的目的:

  • 审查最近在无细胞基因表达系统的进展.
  • 突出CFE对合成生物学应用的变革性影响.
  • 讨论CFE在构建细胞,控制遗传网络和制造生物基产品方面的潜力.

主要方法:

  • 审查CFE系统的最新文献和技术发展.
  • 分析CFE的优势,包括通过冷干燥和固有的生物限制进行分发.
  • 探索合成生物学,基因工程和生物制造中的应用.

主要成果:

  • CFE系统提供精确控制生物分子转换.
  • 冷干燥使CFE系统的易于分发和在使用点进行再水分.
  • CFE的非生物性质比转基因生物体提供了生物控制优势.

结论:

  • CFE系统与传统的基于细胞的方法有很大的不同.
  • 最近的进展扩大了CFE的功能和应用.
  • CFE正在彻底改变生物系统的构建和生物基产品的生产.