相关实验视频

Updated: Jun 4, 2025

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

在一个不朽化的hTERT-bOEC细胞模型中对三个Capripoxvirus感染的比较RNA测序分析

Hongqiang Zhang¹, Fangping Wang², Haotai Chen³

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, PR China; College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, 810016, PR China.

|December 19, 2024

概括

研究人员开发出了一种新的不朽细胞系hTERT-bOEC,对capripoxvirus (CaPVs) 非常敏感. 这一突破有助于对块性皮肤病,绵羊和山羊的研究,这对疫苗开发和了解CaPV病原体至关重要.

科学领域:

兽医病毒学兽医病毒学
细胞生物学细胞生物学
分子病毒学分子病毒学

背景情况:

卡普里波克病毒 (CaPVs) 在牲畜中造成重大经济损失.
了解CaPV病原体需要适合的细胞模型用于研究和疫苗开发.

研究的目的:

建立一种对CaPVs敏感的新型不朽细胞系.
描述新细胞系对研究CaPV感染的适用性.

主要方法:

使用人类端粒酶逆转录酶 (hTERT) 建立了一个不朽的绵羊气管上皮细胞系 (hTERT-bOEC).
通过Westernblotting,免疫光和流细胞计验证了hTERT的整合.
评估细胞系对块性皮肤病病毒 (LSDV),绵羊天花病毒 (SPPV) 和山羊天花病毒 (GTPV) 的敏感性.
进行RNA测序 (RNA-seq) 和定量PCR (qPCR) 来分析基因表达变化.

主要成果:

永久化的hTERT-bOEC细胞系成功建立并确认表达hTERT.
hTERT-bOECs对LSDV,SPPV和GTPV感染具有很高的敏感性.
转录组分析揭示了基因表达模式和通路丰富,类似于体内CaPV感染.
通过使用qPCR识别和验证了关键的差异表达基因.

结论:

永久化的hTERT-bOEC细胞系是CaPV研究的一个有价值的工具.
这一细胞系促进了对CaPVs的基础研究,临床应用和疫苗开发.
转录组数据提供了对CaPV复制机制的见解.

关键词:

卡普里波克病毒感染.不同表达的基因.不朽化不朽化不朽化不朽化在RNA-Seqq.这就是HTERT-bOECEC.

更多相关视频

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Published on: April 13, 2009

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

相关概念视频

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...