葡萄植物中的有机酸的分析和可视化,通过脱电喷雾电离成像
Xiaxia Wang1, Sujin Zheng1, Pietro Franceschi2
1College of Horticulture, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350007, China.
Food chemistry
|December 19, 2024
概括
这项研究使用DESI成像绘制了葡萄树中的有机酸的地图,揭示了和葡萄酸的独特组织特异分布. 这些发现增强了我们对葡萄酒代谢和化学分析的理解.
科学领域:
- 植物科学 植物科学
- 代谢学 代谢学 代谢学
- 农业化学 农业化学
背景情况:
- 有机酸对葡萄和葡萄酒的质量至关重要.
- 有限的研究存在于葡萄植物内有机酸的空间分布.
- 了解这种分布是葡萄藤生理学和果实发育的关键.
研究的目的:
- 在不同的葡萄组织中对关键有机酸进行分析和成像.
- 为了研究葡萄糖和酸的半定量分布.
- 为了揭示在葡萄树内有机酸局部化的空间模式.
主要方法:
- 采用了溶解电喷离电离 (DESI) 成像技术,用于快速化学分析.
- 分析了各种植物组织中酸和酸的比率.
- 利用DESI的半定量能力进行空间分析.
主要成果:
- 观察到果酸和酸的空间分布不同.
- 酸主要在皮质和表皮中发现.
- 葡萄酒酸集中在茎,结节和叶子静脉中,表明组织特异性合成.
结论:
- DESI成像为植物中的半定量化学数据提供高空间分辨率.
- 观察到的酸分布模式表明组织特异性的生物合成活动.
- 这项研究有助于我们更好地了解葡萄中有机酸代谢的过程.
更多相关视频
10:13Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Published on: November 12, 2016
9.8K
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
3.7K
相关概念视频
IR and UV–Vis Spectroscopy of Carboxylic Acids
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
Spectroscopy of Carboxylic Acid Derivatives
Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and unsymmetrical carbonyl vibration.
In the...
In the...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
