通过pH值转移处理来改变蛋黄蛋白结构以进行界面重组:提高溶解度以增强油水界面吸附和乳化特性
Yaqin Yang1, Haobo Jin2, Bao Chen2
1National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan 430070, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China.
Food chemistry
|December 19, 2024
概括
性pH转移处理通过改变蛋白质结构和改善油水界面吸附来增强蛋黄蛋白 (EYP) 乳化. 这优化了EYP作为食品乳化剂的使用.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 体科学 体科学 体科学
背景情况:
- 蛋黄蛋白 (EYP) 是一种有价值的乳化剂.
- 了解EYP在不同条件下的行为对于其应用至关重要.
- 改变pH值的治疗方法可以改变蛋白质的功能.
研究的目的:
- 研究pH转移处理中的不同性如何影响EYP乳化.
- 为了阐明EYP的油水界面吸附机制.
- 为了优化EYP作为食品乳化剂.
主要方法:
- 蛋白质在不同的度水平下接受了pH值转移的处理.
- 测量了蛋白质结构,颗粒大小,度和溶解度的变化.
- 分析了油水界面吸附,界面压力和粘弹性模量.
- 评估了乳液稳定性和化指数.
主要成果:
- 度增加暴露了疏水性群体和改变了EYP的三级结构.
- 降低溶液颗粒大小和蛋黄颗粒分解的pH变化.
- 在pH值12.0时观察到最佳溶解度和最小度.
- 在pH值9.0的最大扩散率与最小颗粒大小相关.
- 在pH12.0的最大界面压力和粘弹性模量使乳液稳定性提高了23.82%.
结论:
- 性pH转移处理显著改善了EYP的乳化特性.
- 该研究确定了最佳的pH条件,以提高EYP的功能.
- 研究结果支持使用EYP作为有效的食品乳化剂.
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