一个可行的调节策略对抗生素耐药性基因的结合,基于不同的细菌定数感应抑制方法
Chen-Yi Jiang1, Xiao-Chi Feng1, Hong-Tao Shi1
1State Key Laboratory of Urban Water Resource and Environment, School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, 518055, PR China.
Water research
|December 19, 2024
概括
多数感应抑制剂通过抑制等离子体结合,有效地减少了抗生素耐药性基因的传播. 这项研究突出了控制环境中抗生素耐药性基因传播的新策略.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 抗生素耐药性基因 (ARG) 在全球范围内传播,等离子体介导的结合是主要的驱动因素.
- 质量感应 (QS) 是一种细胞-细胞通信系统,它调节细菌群体行为,并可能影响结合.
研究的目的:
- 调查QS在促进等离子体结合中的作用.
- 评估QS抑制剂抑制ARG传播的疗效.
- 阐明QS抑制剂影响结合的机制.
主要方法:
- 研究了QS信号对RP4等离子体结合频率的影响.
- 测试了QS抑制剂2(3H) -黄和乙酶I,以测试它们的抑制作用.
- 研究了抑制剂对细胞接触,转移通道形成和基因表达 (sdiA,结合性 pili 基因) 的影响.
- 在活性污泥反应堆中评估了抑制剂的有效性.
主要成果:
- QS抑制剂2(3H) -黄和乙酶I显著抑制RP4等离子体结合频率 (分别为0.32倍和0.13倍).
- 抑制剂降低了细胞接触和转移通道的形成,降低了sdiA基因表达的调节.
- 结合性 pili 形成的基因表达被下调,表明抑制交配对的形成.
- 在活性污泥中,抑制剂实现了84.07%和66.05%的等离子体扩散抑制.
结论:
- QS抑制剂提供了一种可行的策略,以控制抗生素耐药性基因的等离子体介导传播.
- 准QS信号通路,特别是交配对的形成,可以有效地抑制结合.
- 定数灭方法为管理环境环境中ARG传播提供了一个有希望的方法.
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