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Updated: Jun 4, 2025

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

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在细胞中通过LC-MS增强检测化代谢产物的基于Diazonium的衍生

Yikang Wang1, Feifei Lin2, Guozheng Zhu1

  • 1School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310058, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.

Journal of pharmaceutical and biomedical analysis
|December 19, 2024
PubMed
概括

研究人员开发了一种使用DMQX和LC-MS的新方法,用于准确量化化代谢物,这对于理解新陈代谢和疾病至关重要. 这一突破有助于研究代谢机制和相关疾病.

关键词:
染色学分离的分离方法衍生化 衍生化 衍生化这就是LC-MS.化代谢产物化代谢产物定量分析是一种量化分析.

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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