Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.6K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

smDeepFLUOR: single-molecule deep learning fluorescence classification.

Nature communications·2026
Same author

Single-trajectory Bayesian modeling reveals multi-state diffusion of the MSH sliding clamp.

NPJ systems biology and applications·2026
Same author

Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.

Nature communications·2026
Same author

Structural and dynamic basis of Ssp4-mediated DNA protection in foodborne bacterial spores.

Scientific reports·2025
Same author

DNA Hanger: Surface-Minimized Single-Molecule Immunoassay Platform.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Conformational flexibility of His200 enables catalytic activity in the T200H mutant of carbonic anhydrase II.

Molecules and cells·2025
Same journal

Epigallocatechin gallate suppresses adipogenesis by targeting tetranectin and inhibiting its endocytosis via modulation of ERK and AKT signaling pathways.

BMB reports·2026
Same journal

Immunoproteasome inhibition protects the retina from Alzheimer's-associated degeneration.

BMB reports·2026
Same journal

Urolithin A improves mitochondrial dysfunction induced by oxidative stress in human dermal papilla cells.

BMB reports·2026
Same journal

Whole genome-based polygenic risk score and structural variation analysis in Korean patients with inflammatory bowel disease.

BMB reports·2026
Same journal

EK-16005, a 2-anilinopyrimidine derivative, inhibits breast cancer progression by regulating myeloid-derived suppressor cells.

BMB reports·2026
Same journal

Immune phenotype-based stratification of colorectal cancer reveals subtype-specific immunotherapeutic opportunities: insights from a Korean patient cohort.

BMB reports·2026
查看所有相关文章

相关实验视频

Updated: Jun 4, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.1K

低温单分子光成像技术

Phil Sang Yu1, Chae Un Kim2, Jong-Bong Lee3

  • 1Department of Physics, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.

BMB reports
|December 19, 2024
PubMed
概括
此摘要是机器生成的。

冷固定可使生物样本保持接近原生状态,用于高分辨率成像. 低温单分子光显微镜提供了更高的准确性和对生物分子动态的洞察力.

更多相关视频

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

相关实验视频

Last Updated: Jun 4, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.1K
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 显微镜的使用方法

背景情况:

  • 冷固定很快将样品结成无形冰,防止结构扭曲.
  • 化学固定可以导致人工物,限制高分辨率成像.
  • 近原生样本的保存对于研究生物分子结构至关重要.

研究的目的:

  • 审查低温单分子光成像技术.
  • 讨论该领域的进展和未来前景.
  • 突出生物分子研究的冷方法的好处.

主要方法:

  • 低温电子显微镜的使用方法
  • 低温光显微镜的显微镜.
  • 在低温下进行单分子光成像.

主要成果:

  • 低温可以改善光体的特性 (寿命,光漂白,信号对噪声).
  • 纳米尺度的分辨率是通过最近的低温技术取得的.
  • 克服客观数值孔径和冷阶段性能方面的局限性.

结论:

  • 低温单分子光成像提供了准确和有洞察力的数据.
  • 超分辨率,光体和人工智能的未来创新将增强生物分子研究.
  • 冷技术对于理解分子动力学和相互作用至关重要.