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干血斑块中的类固醇激素度:毛细血管和静脉血液样本之间的比较

Anouk Olthof¹, Vera H de Kleijne¹, Anita Boelen²

¹Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam UMC, Location University of Amsterdam, Amsterdam Gastroenterology Endocrinology & Metabolism, Meibergdreef 9, Amsterdam 1105 AZ, the Netherlands; Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam UMC, Location Vrije Universiteit Amsterdam, Amsterdam Gastroenterology Endocrinology & Metabolism, De Boelelaan 1117, Amsterdam 1081 HV, the Netherlands.

Clinica chimica acta; international journal of clinical chemistry

|December 19, 2024

概括

在EDTA管中的静脉血液中的干血斑 (DBS) 适合开发类固醇激素的液体染色学-并联质谱法 (LC-MS/MS) 方法. 这种方法为采样提供了一个可行的替代方案,不包括皮质激素分析.

科学领域:

生物化学生物化学
分析化学分析化学
临床实验室科学临床实验室科学

背景情况:

干血斑点 (DBS) 越来越多地用于实验室测量.
开发强大的液体染色学-并联质谱法 (LC-MS/MS) 方法需要大量的DBS样本.
静脉血液在临床环境中很容易获得,可用于准备DBS.

研究的目的:

评估从静脉血液 (采集在EDTA管中) 中制备的DBS适合开发和验证LC-MS/MS方法.
为了确定静脉血液衍生的DBS是否可以与毛细血管DBS相比,可靠地量化类固醇激素.

主要方法:

从20名健康志愿者收集了毛细血管和静脉血液样本 (EDTA和非EDTA管).
从静脉血液样本中制备的体积DBS.
使用内部LC-MS/MS方法分析了类固醇激素 (,安德罗斯,17-OHP,皮质醇,皮质素,皮质,DHEA-S).

主要成果:

来自静脉EDTA管的DBS显示了大多数类固醇激素与毛细血管DBS的高相关性 (≥0.89),除了皮质 (0.67).
来自非EDTA静脉管的DBS与EDTA静脉DBS (≥0.97) 和毛细血管DBS (>0.90) 强烈相关,不包括皮质 (0.64).

结论:

从EDTA管中收集的静脉血液中制备的DBS适合开发和验证大多数类固醇激素的LC-MS/MS方法.
这种方法为使用LC-MS/MS的类固醇激素分析提供了切实可行的替代方案,除了皮质激素定量.

关键词:

雄激素是一种基.皮质类固醇是一种皮质类固醇.在 DBS 中,DBS 是 DBS 的代名词.干燥的血液斑点干燥的血液斑点这是LC-MS/MS.样本矩阵的样本矩阵.

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