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相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.3K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

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In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
465
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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相关实验视频

Updated: Jun 4, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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DiffMAP-GP:使用高斯过程从粒子轨迹进行连续的2D扩散图,而无需使用数据组合.

Vishesh Kumar1, J Shepard Bryan1, Alex Rojewski1

  • 1Center for Biological Physics, Arizona State University, Tempe, Arizona; Department of Physics, Arizona State University, Tempe, Arizona.

Biophysical reports
|December 19, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了使用高斯过程的贝叶斯框架DiffMAP-GP,用于绘制细胞膜上的扩散系数. 这种方法揭示了当地的膜特性,而无需数据对接.

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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

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相关实验视频

Last Updated: Jun 4, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

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科学领域:

  • 生物物理学的生物物理.
  • 计算生物学 计算生物学
  • 膜生物物理学 膜生物物理学

背景情况:

  • 在细胞膜内,扩散系数在空间上有所不同,从而提供了对局部膜性质 (如组成和刚性) 的见解.
  • 量化这些变异对于理解膜动态和功能至关重要.

研究的目的:

  • 开发一个贝叶斯框架,DiffMAP-GP,用于从粒子跟踪数据中量化扩散系数空间地图及其不确定性.
  • 提供一种无监督的方法,在没有数据对接的情况下,在膜上连续映射扩散系数.

主要方法:

  • 在扩散系数图上使用贝叶斯框架与高斯过程 (GP) 先验.
  • 采用诱导点方法来计算效率与非结合的概率前对.
  • 分析膜蛋白质的合成数据和活细胞单分子成像数据.

主要成果:

  • 成功生成了扩散系数及其不确定性的空间地图.
  • 通过已知的基本事实验证了合成数据的框架.
  • 从活细胞成像中获得的真实生物数据的证明适用性.

结论:

  • DiffMAP-GP提供了一种严格的,无监督的方法,可以在膜上连续地映射扩散系数.
  • 该框架通过量化扩散异质性,提供了对局部膜性质的宝贵见解.
  • 这种工具有助于分析用于膜生物物理学研究的粒子跟踪数据.