通过乳糖酶催化通过奇托对印加花生蛋白-多联合物的修饰:结构性,界面性和功能性质
Yishuang Yang1, Ziqi Deng1, Zhanpeng Chen1
1College of Food Science, South China Agricultural University, No. 483, Wushan Street, Tianhe District, Guangzhou 510642, China.
International journal of biological macromolecules
|December 19, 2024
概括
印加花生白蛋白 (IPA) 与素 (CS) 和多的酶交联改善了结构,界面和乳化特性. 这种绿色方法增强了IPA的功能.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 酶交联提供了一种绿色方法来修改蛋白质和多糖.
- 奇托 (CS) 和印加花生专 (IPA) - - 聚是有潜在应用的有价值的生物聚合物.
- 了解酶催化交联对IPA-多联的结构影响对于优化其功能至关重要.
研究的目的:
- 为了研究IPA-多联体在与CS的乳酶催化交联时的结构变化.
- 评估由此产生的共聚物质的界面,乳化和抗氧化特性.
- 评估这些共聚物在高内部相皮克林乳液 (HIPEs) 中的性能.
主要方法:
- 使用laccase的方法将IPA-酸 (GA) 和IPA-(-) -epigallocatechin-3-gallate (EGCG) 与奇托进行酶性交联.
- 分析结构变化,使用循环二元化等技术来评估二次结构 (α螺旋,β螺旋).
- 测量接触角度以确定疏水性和界面吸附.
- 使用合成的共聚合物作为乳化剂,制备和表征高内部相皮克林乳液 (HIPEs).
- 评估乳化,质性 (粘度,粘弹性) 和抗氧化活性.
主要成果:
- 酶交联减少了α-螺旋体和增加了β-螺旋体,导致IPA-GA-CS和IPA-EGCG-CS中更有序的结构.
- 接触角度显著降低,表明疏水性降低和界面吸附增强.
- 由共聚合物稳定的HIPEs表现出改善的乳化和抗氧化特性.
- IPA-GA-CS共聚合物显著提高了HIPE的表面粘度和粘弹性.
- EGCG诱导的共聚物表现出优越的脂质抗氧化能力.
结论:
- 蛋白质多多糖合物聚合物 (IPA-polyphenol-CS) 的乳糖酶介导的交联是一种提高功能性质的有效策略.
- 修改后的共聚物表现出改善的界面,乳化和抗氧化活性,适合HIPE稳定.
- 这种新的蛋白质修饰方法增强了IPA的功能性质和抗pH敏感性,为生物聚合物应用开辟了新的途径.
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