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Updated: Jun 26, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
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An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

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为宿主细胞蛋白质表征开发一个优化的LC-MS工作流程,以支持上游过程开发

Janik D Seidel1, Mark R Condina1,2, Manuela Klingler-Hoffmann1

  • 1Clinical and Health Sciences, University of South Australia, 5000 Adelaide, Australia.

Journal of proteome research
|December 19, 2024
PubMed
概括

这项研究引入了一种更快的质谱法 (MS) 方法,用于在生物治疗中识别和量化1000多种宿主细胞蛋白 (HCP). 这种改进的分析协议增强了生物制药生产的质量控制.

关键词:
中国仓鼠的卵巢这就是LC-MS.绝对量化的绝对量化.生物处理生物处理.澄清的细胞培养液体数据独立的获取采集.hi3量化定量化的方法宿主细胞蛋白质是宿主细胞的蛋白质.过程分析技术 过程分析技术

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Author Spotlight: Detecting Low-Abundant Host Cell Proteins in Drug Products Using Enrichment Beads and Limited Digestion09:45

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Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...