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[Epimedium黄类微粒对肺前转移性微环境的调节作用]

Xiao-Yu Che¹, Meng-Ting Xu¹, Ya-Qin Li¹

¹Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine Nanjing 210028, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

|December 19, 2024

概括

这项研究表明,抑制干扰素基因刺激器 (STING) 信号减少了转移前的微环境和肺转移. 总Epimedium黄酸盐 (EF) 微粒通过干预这种炎症环境,显示出控制瘤扩散的潜力.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
纳米医学是一种纳米医学.

背景情况:

瘤转移是癌症相关死亡的主要原因.
转移前微环境假设为理解转移提供了一个新的框架.
向干扰素基因刺激器 (STING) 信号传递是一种抑制转移的潜在策略.

研究的目的:

研究STING信号在调节前转移性微环境中的作用.
为了评估通过微粒传递的总Epimedium黄酸盐 (EFs) 的抗转移潜力.
评估STING干扰对肺转移的影响.

主要方法:

使用复合腺相关病毒载体介导的STING短毛RNA干扰 (rAAV STING shRNA) 构建一个前转移和肺转移模型.
来自易斯肺癌细胞的EFs-LLC-MP微粒的制备和表征.
评估STING表达,前转移性微环境标记物 (纤维素,lysyl氧化酶) 和肺转移,使用西斑,Micro-CT,HPLC和流细胞计.

主要成果:

通过鼻子注射RAAV STING shRNA显著抑制了STING的表达,并减少了前转移性微环境标记物.
微型CT显示,在STING干扰后,肺转移和结节的减少.
EFs-LLC-MPs被膜上皮细胞和巨细胞有效地内化,减少了转移前标志物的表达.

结论:

刺痛信号在肺部转移前微环境的形成中起着至关重要的作用.
总EFs微粒成功制备,并证明其在调节炎性转移前微环境方面的潜力.
这些发现表明,使用EFs载微粒来控制瘤转移是一种有前途的治疗策略.

关键词:

埃皮米迪乌姆的黄类化合物细胞外囊泡中的细胞外囊泡.肺前转移性微环境干扰素基因的刺激剂.瘤转移的发生.

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