相关实验视频

Updated: Jun 4, 2025

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

一个染色体层次的基因组组合的大巴贝尔精神分裂症,精神分裂症巨巨

Xuanguang Liang¹, Wenhao Wang¹, Junrou Huang¹

¹School of Marine Sciences, Sun Yat-sen University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519082, Guangdong, China.

Scientific data

|December 20, 2024

概括

我们使用先进的测序技术组装了易受伤害的大巴贝尔精神分裂症 (Schizothorax macropogon) 的染色体规模基因组. 这种基因组资源有助于保护这种特有鱼类和相关的精神分裂症患者的保护工作.

科学领域:

基因组学就是基因组学.
保护生物学保护生物学
鱼类学鱼类学鱼类学

背景情况:

大巴巴鱼 (Schizothorax macropogon) 是一种在Yarlung Zangbo河中发现的特有和脆弱的鱼类物种.
这种物种面临着由于其恶劣环境中的人为压力而导致的种群减少.

研究的目的:

为了生成一个高质量的,染色体规模的基因组组合,用于Schizothorax macropogon.
提供一个基因组资源,以了解石鱼的进化和生态.
支持Schizothorax macropogon和相关物种的保护战略.

主要方法:

利用PacBio,MGI-Seq和Hi-C测序数据进行基因组组装.
组装了一个染色体规模的基因组,包括25个伪染色体.
使用BUSCO.进行基因注释和评估基因组完整性.

主要成果:

基因组组件的大小为1.42 Gb,支架N50为59.4 Mb,表明高度连续性.
97.9%的BUSCO得分证实了大会的全面性.
鉴定了46,246个编码蛋白质的基因,其中49.61%的基因组由重复序列组成.

结论:

染色体尺度基因组组合的斯基索托拉克斯macropogon是一个重要的成就.
这种基因组资源将有助于研究共鱼的进化历史和生态适应.
这次会议对于制定有效的保护计划对这种脆弱的特有物种至关重要.

更多相关视频

2D and 3D Chromosome Painting in Malaria Mosquitoes

2D and 3D Chromosome Painting in Malaria Mosquitoes

Published on: January 6, 2014

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

相关概念视频

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.