通过in vitro和in silico方法评估Tridax procumbens二次代谢物的抗结核活性
Jayashri Seetharaman1, R Akshaya Priya1, Reya Rene Philip1
1Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, 603203, India.
Current microbiology
|December 20, 2024
概括
这项研究研究了药用植物Tridax procumbens对抗Mycobacterium tuberculosis的活性. 结果显示,Tridax procumbens 具有抗菌菌性质,并有可能作为结核病的天然治疗方法.
科学领域:
- 植物化学 植物化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 结核病 (TB) 仍然是由Mycobacterium tuberculosis引起的全球重大健康挑战.
- 传统的药用植物对病原体有希望,但抗菌细菌研究是有限的.
- 研究新的植物衍生化合物对于开发新的抗结核病疗法至关重要.
研究的目的:
- 为了确定Tridax procumbens的植物化学成分.
- 评估Tridax procumbens对Mycobacterium tuberculosis H37Rv的抗菌和抗菌细菌活性.
- 阐明其活性化合物的作用机制.
主要方法:
- 对抗菌活性进行磁盘扩散方法.
- 微板Alamar蓝色试验 (MABA) 和BACTEC MGIT 960 DST对抗菌菌活性进行检测.
- 用于植物化学分析和作用机制的MTT测定,FTIR,GC-MS和分子对接.
主要成果:
- 特里达克斯前表明抗菌菌的活性,最小抑制度 (MIC) 为600μg/mL,对H37Rv.
- 植物提取物被发现易受H37Rv和耐利芬素 (RR).
- 分子对接确定了 (S,Z) -Heptadeca-1,9-dien-4,6-diyn-3-ol作为一种关键化合物,与 gyrA 基因具有强烈的结合亲和力.
结论:
- 特里达克斯普罗坎本斯具有针对Mycobacterium tuberculosis的显著抗菌特性.
- 这项研究支持Tridax procumbens作为新型抗结核病药物发现的来源的潜力.
- 对其活性化合物和机制的进一步研究是有必要的.
更多相关视频
10:29A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
7.8K
09:57Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
Published on: December 14, 2016
10.4K
相关概念视频
01:26Pulmonary Tuberculosis IV
133
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
133
01:28Pulmonary Tuberculosis V
170
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
170