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相关概念视频

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.5K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.7K
Homologous Recombination02:31

Homologous Recombination

50.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.2K
CRISPR01:59

CRISPR

49.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.3K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

28.7K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
28.7K

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相关实验视频

Updated: Jun 4, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

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ncRNA编辑:功能性表征和计算资源

Gioacchino P Marceca1, Giulia Romano2, Mario Acunzo2

  • 1Independent Researcher, Vittoria, RG, Italy.

Methods in molecular biology (Clifton, N.J.)
|December 20, 2024
PubMed
概括
此摘要是机器生成的。

腺至氨酸 (A-to-I) RNA编辑动态修改非编码RNA (ncRNA),影响基因调节和疾病. 这种编辑在癌症和其他疾病中至关重要,具有作为生物标志物和治疗点的潜力.

关键词:
3′UTR 时间一到一的RNA编辑.生物信息学是一种生物信息学.数据库 数据库就是数据库.这里面是Introns.没有NGS,没有NGS.在ncRNA中,我们可以这是一个小RNARNA.编辑ncRNA编辑

更多相关视频

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
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相关实验视频

Last Updated: Jun 4, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 非编码RNAs (ncRNAs) 是基因表达,转化和疾病发病的重要调节者,特别是在癌症中.
  • ncRNAs被广泛分为短和长形式,每个都有不同的调节作用.
  • 氨酸到氨酸 (A-to-I) RNA编辑是一种转录后修改,改变RNA序列和结构.

研究的目的:

  • 探索A-to-IRNA编辑在短 (微RNA) 和长ncRNA中的功能意义.
  • 要突出RNA编辑对ncRNA生物发生和功能的影响.
  • 讨论RNA编辑在疾病发展中的作用及其作为治疗点的潜力.

主要方法:

  • 审查关于ncRNA编辑的当前文献.
  • 整合生物信息学资源用于ncRNA组研究.
  • 讨论下一代测序 (NGS) 技术用于全基因组RNA编辑检测.

主要成果:

  • 编辑miRNA与促进质母细胞瘤侵袭性有关.
  • 在各种癌症类型中对RNA编辑热点的表征.
  • RNA编辑失调与甲状腺癌,缺血和其他人类疾病有关.

结论:

  • A-to-I RNA编辑动态调节ncRNA功能,影响疾病的进展.
  • RNA编辑机制的失调为开发新生物标志物和治疗策略提供了机会.
  • 使用先进的计算工具和NGS进行全面的ncRNAome研究对于了解RNA编辑的生物和临床影响至关重要.