AMPK通过降低P-HOG1 MAPK水平来激活Penicillium funiculosum中的细胞质分泌物
Anmoldeep Randhawa1,2, Tulika Sinha1, Maitreyee Das1
1Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Journal of basic microbiology
|December 20, 2024
概括
在真菌中,AMP激活蛋白激酶 (AMPK) 对于细胞酶分泌至关重要,而不是生产. 它在碳稀缺期间充当全球抑制剂,通过依赖的途径控制分泌,维持细胞平衡.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 在营养稀缺的情况下,细胞酶的生产对线状真菌来说是能源密集的.
- AMP激活蛋白激酶 (AMPK) 是营养和能量水平的关键真核细胞传感器.
- 尽管已知AMPK在碳利用中的作用,但AMPK在细胞酶合成中的作用尚未完全理解.
研究的目的:
- 为了研究AMPK在Penicillium funiculosum细胞酶合成中的精确作用.
- 阐明在碳压力下细胞质生产和分泌的调节机制.
主要方法:
- 对snf1基因的基因删除分析.
- 定量蛋白质组学分析蛋白质表达和局部化.
- 化学遗传方法和西方斑点分析来研究信号通路.
主要成果:
- AMPK (snf1) 对于细胞酶分泌至关重要,而不是转录或翻译,以一种依赖的方式.
- 删除snf1导致细胞内细胞酶的积累和HOG1 MAPK通路的激活.
- 一个信号/激活的AMPK通路调节了一个涉及化HOG1 (P-HOG1) 的分泌检查点.
结论:
- 在碳压力期间,AMPK充当全球抑制剂,维持细胞平衡.
- AMPK通过涉及HOG1 MAPK通路的依赖机制调节细胞酶分泌.
- 扰乱P-HOG1/HOG1 MAPK平衡阻断细胞酶分泌,突出其作为分泌检查点的作用.
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