形态依赖的纳米塑测定:用于多重总抗氧化能力分析的强大信号平台
Zeinab Feyzollahi1, Mahdiye Hassanpoor1, Afsaneh Orouji1
1Department of Chemistry, Sharif University of Technology, Tehran, 111559516, Iran. hormozi@sharif.edu.
Analytical methods : advancing methods and applications
|December 20, 2024
概括
一个新的多色度测试探头提供了一种无毒的,用户友好的方法来评估唾液的总抗氧化能力 (TAC). 这种创新的传感器可以准确地在现场监测健康和疾病预防的关键抗氧化剂.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 纳米技术纳米技术
背景情况:
- 总抗氧化能力 (TAC) 对健康至关重要,反映了身体对抗氧化应激的防御能力.
- 唾液抗氧化剂,如亚酸 (AA),氨酸 (CYS),谷氨酸 (GSH) 和尿酸 (UA) 表示系统水平.
- 需要强大的,无毒的传感器来监测唾液TAC以进行健康评估.
研究的目的:
- 开发一种创新的多色度探针,用于高分辨率,肉眼检测唾液TAC.
- 利用等离子体纳米结构,精确有效地分析单个和总唾液抗氧化剂.
- 为了验证探针在现场进行非侵入性健康监测的有效性.
主要方法:
- 一个基于等离子纳米结构的多色度探针被设计出来.
- 模式识别和回归分析用于抗氧化剂的识别和量化.
- 使用纯抗氧化剂,TAC混合物和真实唾液样本来评估探针的性能.
主要成果:
- 探测器显示了AA,CYS,GSH,UA和TAC混合物的线性反应和低检测极限.
- 通过性能指标和真实唾液样本的验证证实了强度和有效性.
- 独特的反应概况允许基于内源性抗氧化剂比率进行精确的TAC量化.
结论:
- 开发的多色度测量探头是一种快速,经济高效,用户友好和无毒的工具,用于现场唾液 TAC 监测.
- 这种方法促进了抗氧化剂的全面分析,支持健康监测和疾病预防.
- 探针能够精确量化唾液中的TAC,这对于临床诊断非常适用.
相关概念视频
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
01:13Mass Analyzers: Overview
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
01:19Mass Analyzers: Common Types
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...