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相关概念视频

RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

10.8K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.8K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Protein Modifications in the RER01:26

Protein Modifications in the RER

5.0K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.0K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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相关实验视频

Updated: Jun 4, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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多模式的CLIP告知蛋白质编辑

Mingze Yin1, Hanjing Zhou2, Yiheng Zhu2

  • 1School of Medicine, Zhejiang University, Hangzhou, China.

Health data science
|December 20, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了ProtET,这是一种新的机器学习方法,用于使用生物文本指令编辑蛋白质. 这种方法有效地增强了蛋白质的功能,如稳定性和结合性,推进了人工蛋白质的设计.

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Last Updated: Jun 4, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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科学领域:

  • 生物技术是生物技术.
  • 计算生物学 计算生物学
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 蛋白质对生物功能至关重要,可控制的蛋白质编辑有助于研究和治疗.
  • 机器学习辅助蛋白质编辑 (MLPE) 加快了优化,但在巨大的序列空间和直接的人类反集成方面存在困难.
  • 现有的方法缺乏基于自然语言指令进行蛋白质编辑的能力.

研究的目的:

  • 引入 ProtET,一种高效的方法,用于使用多模式学习进行 CLIP 信息的蛋白质编辑.
  • 通过生物文本指令引导蛋白质编辑,增强人类的互动性.
  • 为了克服对蛋白质序列生成的组合空间探索的局限性.

主要方法:

  • ProtET采用两阶段的方法:预训练与对比学习,通过大型语言模型 (LLM) 调整蛋白质和生物文本表示.
  • 蛋白质编辑阶段融合了编辑说明和原始蛋白质序列的特征.
  • 生成的目标蛋白序列在这些融合特征上进行条件化,以便精确编辑.

主要成果:

  • ProtET在编辑蛋白质方面表现出卓越的性能,以提高酶活性,稳定性和抗体结合的功能.
  • 该方法显著提高了蛋白质的稳定性,获得了16.67%和16.90%的收益.
  • ProtET的性能远远超过了现有的最先进的方法.

结论:

  • ProtET提供了一种强大的新功能,用于以自然语言为指导的人工蛋白质编辑.
  • 这种进步有可能解决学术,工业和临床应用中的未满足需求.
  • 这种方法有助于创建具有所需功能的新型蛋白质结构.