卡哈尔体标记蛋白林是SUMOylated的,具有类似SUMO E3酶的活性
Katheryn E Lett1, Douglas M McLaurin1, Sara K Tucker1
1Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
概括
在Cajal体中发现的蛋白质Coilin通过影响DGCR8酸化来调节microRNA生物发生,并促进蛋白质SUMOylation,影响细胞稳定性和基因表达.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 体 (CBs) 对于核糖蛋白生物生成至关重要,但其标记蛋白质 - - 林的功能在缺乏丰富的CBs的细胞中尚不清楚.
- 已知Coilin通过化DGCR8来促进microRNA生物发生,DGCR8是微处理器复合体的关键组成部分.
- 蛋白质稳定性也受到SUMOylation等翻译后修饰的调节.
研究的目的:
- 研究可林在DGCR8SUMOylation中的作用及其对蛋白质SUMOylation的更广泛影响.
- 阐明coilin在调节microRNA生物发生和蛋白质稳定中的机制.
- 为了确定可林是否具有类似于SUMO E3酶的活性.
主要方法:
- 使用了可林敲击和敲击细胞系.
- 对DGCR8,Sp100,SMN和RanGAP1的酸化和SUMOylation水平进行了评估.
- 研究了Coilin与SUMOylation机械组件 (Ubc9,SUMO2) 的相互作用.
- 在体外和体内进行了SUMOylation测试.
主要成果:
- 林倒置降低了7个额外地点的DGCR8酸化,并降低了包括DGCR8,Sp100和SMN在内的全球蛋白质SUMOylation.
- 林表达挽救了Sp100 SUMOylation,并在林淘汰细胞中增加了DGCR8和SMN水平.
- 林促进RanGAP1的SUMOylation,与Ubc9和SUMO2相互作用,并且本身也是SUMOylated的.
结论:
- 林被认为是DGCR8酸化的调节剂.
- 林促进蛋白质SUMOylation,表现出类似SUMO E3酶的活性.
- 这些发现凸显了可林在调节基因表达和蛋白质平衡中的多方面的作用.
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