相关实验视频

Updated: Jun 4, 2025

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

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5'终端核酸决定了IVTRNAs的免疫性

Magdalena Wolczyk1, Jacek Szymanski1, Ivan Trus1

  • 1International Institute of Molecular and Cell Biology in Warsaw, Ksiecia Trojdena 4, 02-109 Warsaw, Poland.

Nucleic acids research
|December 20, 2024
PubMed
概括

在体外转录 (IVT) 中启动核酸对RNA免疫性产生影响. 以5'-三酸腺 (5'-pppA) 开始的RNA比以5'-三酸关 (5'-pppG) 开始的RNA更具有免疫性,这是由于增加了双链RNA (dsRNA) 生产.

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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA06:46

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

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Initiation of Translation02:33

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
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Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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pre-mRNA Processing

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Nucleic Acid Structure

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DNA Structure
DNA...
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Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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