相关实验视频

Updated: Jun 4, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

一个基于pyrazoline的光探针具有显著的斯托克斯转移,用于检测Cu2+离子及其应用

Yajing Shang¹, Xinghu Wu¹, Haoting Luo¹

¹School of Chemistry and Chemical Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China. shangyaj@mail.lzjtu.cn.

Analytical methods : advancing methods and applications

|December 20, 2024

概括

一种新的pyrazoline衍生物,BBD,有效地检测出具有高选择性和灵敏性的铜离子 (Cu2+). 这种光探针适用于环境水分析和生物成像应用.

科学领域:

分析化学分析化学
化学传感器化学传感器
材料科学材料科学材料科学

背景情况:

铜离子 (Cu2+) 在生物系统中起着至关重要的作用,但高水平可能有毒.
对Cu2+的敏感和选择性检测方法对于环境监测和生物研究至关重要.
皮拉衍生物已经显示出作为离子检测的光探针的潜力.

研究的目的:

为了合成和表征一种新的pyrazoline衍生物 (BBD) 用于对Cu2+的选择性检测.
评估BBD对Cu2+的光物理性质和传感性能.
为了证明BBD探头在现实世界样本和生物成像中的适用性.

主要方法:

合成的pyrazoline衍生品BBD. 的合成.
光谱分析 (光,IR,ESI-MS) 用于表征和机制阐明.
工作图和TD-DFT计算以确认绑定模式和识别机制.
在水样分析和活细胞/斑马鱼成像中的应用.

主要成果:

在EtOH/HEPES缓冲器 (pH7) 中,BBD通过光火对Cu²⁺具有很高的选择性.
探测器显示出一个大的斯托克斯转移 (122 nm),激发在398 nm,发射在520 nm.
为Cu²⁺实现了低检测极限 (0.862μM),远低于世卫组织指南.
在环境水样中检测Cu2+和在生物系统中成像的成功应用.

结论:

合成的pyrazoline衍生品BBD是一种高度选择性和敏感的光探针,用于Cu2+检测.
BBD表现出显著的优势,包括一个大的斯托克斯转移和低检测极限.
探测器在环境和生物环境中的成功应用凸显了其实际使用的潜力.

更多相关视频

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

相关概念视频

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...