相关实验视频

Updated: Aug 10, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

用于功能化聚合物材料的制备,富含水友群,用于蛋白质组和糖蛋白质组在血清中的分析

Xiuqin Sheng¹, Jiakai Chen¹, Jiahui Shao¹

¹Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China. yanyinghua@nbu.edu.cn.

|December 20, 2024

概括

研究人员开发了一种富含的新型聚合物,用于丰富和糖. 这种材料具有很高的灵敏度和选择性,有助于从患者血清中识别潜在的结直肠癌生物标志物.

科学领域:

生物化学生化学
材料科学材料科学材料科学
分析化学分析化学

背景情况:

蛋白质酸化和糖化是疾病发展的关键生物标志物.
研究这些翻译后修改需要先进的丰富技术.

研究的目的:

开发一种富含的新型聚合物,用于高效丰富和糖.
评估材料在敏感性,选择性,承载能力和可回收性方面的性能.
在结直肠癌血清中应用生物标志物发现材料.

主要方法:

1,2-环氧-5-烯,N,N-甲基二甲胺和乙烯酸的聚合,形成PVME.
通过与氨酸的环开反应增强水友性,形成Cys-PVEM.
通过将Cys-PVEM与硫酸结合,形成富含的聚合物 (Cys-PVME-Ti4+).
使用灵敏度,选择性,负载能力和恢复率指标,对Cys-PVME-Ti4+进行和糖丰富的表征.
在结直肠癌患者血清和正常对照中分析蛋白和糖蛋白的材料的应用.

主要成果:

该Cys-PVME-Ti4+聚合物表现出高灵敏度 (0.02 fmol对于类,0.01 fmolμL-1对于糖类) 和选择性 (1:1000对于类, 1:2000对于糖类).
该材料表现出优异的负载能力 (62 mg g-1),可回收性 (9 个循环) 和回收率 (101.6 ± 0.60% 对于类, 98.7 ± 1.2% 对于糖类).
对结直肠癌血清的分析与正常对照组相比,发现了不同的蛋白和糖蛋白配置,其中包括补体激活和血液凝结等途径的差异.

结论:

Cys-PVME-Ti4+是一种高效的材料,用于和糖的丰富.
鉴定出的差异性蛋白质概况表明,它们可能在结直肠癌的发病过程中起作用.
这种材料对生物标志物发现和癌症研究中的诊断应用具有前景.

更多相关视频

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

相关概念视频

Precipitation and Co-precipitation

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...