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Updated: Aug 3, 2026

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

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通过激活诱导标记物和Th1细胞因子检测对人类抗原特异性T细胞的流动细胞计免疫类型鉴定协议

Gianluca Rotta1, Valentina Achille2, Scott J Bornheimer3

  • 1Becton, Dickinson & Company, 20161 Milan, Italy.

STAR protocols
|December 20, 2024
PubMed
概括

本研究详细介绍了一种流细胞计方案,用于识别使用激活诱导标记 (AIM) 和Th1细胞因子检测的抗原特异性多功能T细胞,用于适应性免疫研究.

关键词:
细胞生物学 细胞生物学细胞培养培养的细胞培养基于细胞的测试流动细胞计量 (Flow Cytometry) 是一种流动细胞计.免疫学 免疫学 免疫学

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...