两种类似氨基酸载体基因对土豆生长的影响
Chao Zhang1, Mingying Shi2, Yuquan Lin2
1Shaanxi Institute of Bio-Agriculture, Xian, Shaanxi, 710043, People's Republic of China; Huzhou Wuxing Jinnong Ecological Agriculture Development Co. LTD, Huzhou, Zhejiang, 313000, People's Republic of China; School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu, Zhejiang, 221116, People's Republic of China.
Journal of plant physiology
|December 20, 2024
概括
马氨基酸载体 StAAP1 和 StAAP8 影响植物生长. 过度表达StAAP8增加了中的氨基酸含量,影响了土豆的营养和蛋白质合成.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 氨基酸运输体 (AAT) 是关键的膜蛋白,促进氨基酸在细胞膜中的运输,对植物生长和发育至关重要.
- 氨基酸浸透酶 (AAP) 子家族,是激活转录因子家族的一部分,代表了AATs的关键组.
- 了解土豆AAP基因的特定作用对于提高作物产量和营养价值至关重要.
研究的目的:
- 在土豆 (Solanum tuberosum) 中识别和描述AAP类基因.
- 为了研究特定的土豆AAP基因StAAP1和StAAP8的亚细胞定位和功能作用.
- 评估 StAAP1 和 StAAP8 过度表达对土豆植物生长,茎发育和氨基酸积累的影响.
主要方法:
- 生物信息分析被用来识别土豆中的八个AAP类基因.
- 构建了绿色光蛋白 (GFP) 表达载体,以确定StAAP1和StAAP8.的血膜定位.
- 过度表达载体是为了生成转基因土豆植物进行表型和生物化学分析而创建的.
主要成果:
- 八个土豆AAP类基因根据孔隙层残留物差异被分为两个子组.
- 亚细胞定位证实StAAP1和StAAP8是与血膜结合的蛋白质.
- 过度表达 StAAP1 和 StAAP8 增强了叶子生长和叶面积; StAAP1 特别延长的茎.
- 过度表达StAAP8显著增加了土豆茎中的氨基酸含量,特别是那些参与蛋白质合成的土豆茎.
结论:
- StAAP1和StAAP8在土豆生长中发挥着不同的作用,StAAP1影响形态,两者都影响了叶子的发育.
- StAAP8与促进土豆中的氨基酸积累有关,这表明它在提高营养质量方面发挥了作用.
- 这些发现为未来的研究提供了基础,通过对氨基酸载体的基因操纵来优化土豆的营养.
相关概念视频
01:27Glucose Transporters
22.4K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.4K
01:06Carrier-Mediated Transport
254
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
254
01:31ABC Transporters: Exporter
4.1K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
4.1K
02:50Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
01:18Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
01:27ABC Transporters: Importer
2.7K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
2.7K