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Updated: Jun 4, 2025

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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在细菌中通过PAM独立的基因编辑进行一次性基因失活

Xin Li1, Ying Wei1, Shu-Yan Wang1

  • 1School of Environmental Science and Engineering, Shandong University, Qingdao, China.

The Journal of biological chemistry
|December 20, 2024
PubMed
概括

我们开发了XSTART,这是一个新的基编辑系统,可以在没有DNA断裂的情况下使细菌基因失活. 这种不受约束的系统实现了近100%的效率,并且在各种细菌物种中起作用.

关键词:
克里斯普里斯卡斯这就是Spry.细菌 细菌 细菌是一种细菌.基础编辑 基础编辑基因失活是基因失活的一种.

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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells11:35

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