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马尼拉海 (Ruditapes philippinarum) 的托尔类受体4 (TLR4) 的识别和功能分析

Xianbo Qiu¹, Jianfeng Ding¹, Yuhang Wang¹

¹College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China.

International journal of biological macromolecules

|December 20, 2024

概括

马尼拉海中的收费类受体4 (TLR4) 在Vibrio parahaemolyticus感染期间促进细胞亡. 这项研究揭示了RPTLR4的价值.

科学领域:

免疫学免疫学免疫学
海洋生物学海洋生物学
分子生物学分子生物学

背景情况:

收费类受体4 (TLR4) 对于对病原体的天生的免疫活性至关重要.
对于TLR4在软体动物亡和对抗细菌感染的宿主防御中的作用尚不清楚.

研究的目的:

克隆和表征马尼拉Ruditapes philippinarum的收费类受体4 (RpTLR4). 为了克隆和表征马尼拉的收费类受体4 (RpTLR4).
研究RpTLR4在马尼拉免疫反应中的作用,特别是由Vibrio parahaemolyticus诱导的亡.

主要方法:

克隆和RpTLR4.4的序列分析.
使用qRT-PCR进行组织表达分析.
亚细胞局部化研究.
在Vibrio parahaemolyticus挑战后通过qRT-PCR和西方抽样进行RPTLR4表达分析.
通过RpTLR4过度表达和沉默实验进行功能分析.

主要成果:

克隆了RpTLR4,揭示了保存的TIR域和与其他软体动物TLR4s有密切的遗传关系.
RpTLR4mRNA在血细胞和中表达高,并且局部存在细胞膜中.
在Vibrio parahaemolyticus感染后,RpTLR4表达显著上调.
过度表达RpTLR4增加了细胞亡,而沉默RpTLR4减少了V. parahaemolyticus诱导的血细胞亡.

结论:

RpTLR4在马尼拉的防御中扮演着关键的亲细胞灭绝作用,抵御Vibrio parahaemolyticus感染.
RpTLR4调解血细胞中的亡,有助于软体动物的宿主免疫力.
这项研究为软体动物对抗细菌病原体的天生的免疫机制提供了基础的见解.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.马尼拉是什么意思马尼拉是什么意思托尔类受体 (TLR4) 是一种类似于托尔类受体.这种病毒是Vibrio parahaemolyticus.

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