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通过Streptomyces coeruleorubidus NRRL B-256969对2'-基黄的特定化和葡萄糖化

Jie Ren¹, Kyle Jackson¹, Caleb Don Barton¹

¹Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105, USA.

Journal of bioscience and bioengineering

|December 20, 2024

概括

这项研究生物合成了新的黄类衍生物,提高了水溶性,改善了抗氧化和抗癌活性. 菌株Streptomyces coeruleorubidus NRRL B-2569有效地修改了黄类化合物,以提高生物可用性和生物活性.

科学领域:

自然产品化学自然产品化学
生物技术是生物技术.
药理学药理学是指药理学的学科.

背景情况:

黄酸具有多样化的生物活性,但其水溶性和生物可用性较差.
提高黄酸盐的溶解度和生物可用性对于实现其充分的健康潜力至关重要.

研究的目的:

生物合成具有改善物理化学和生物特性的新型黄类衍生物.
为了评估*Streptomyces coeruleorubidus*NRRL B-2569在修改黄类药物的疗效.

主要方法:

使用*Streptomyces coeruleorubidus*NRRL B-2569.9*从2'-基黄中生物合成黄衍生物.
使用1D和2DNMR光谱学对新化合物的结构特征.
对水溶性,抗氧化活性 (DPPH测定) 和对质母细胞干细胞的抗癌活性进行评估.

主要成果:

四种衍生物被生物合成;三种是新型结构 (化合物 3-5).
化合物3-5的水溶性显著提高 (36-340倍增加).
化合物3对GSC33.33的抗氧化活性有所改善 (逻辑IC50从10.77 μM降至9.55 μM) 和强大的抗癌作用 (IC50从25.05 μM降至7.07 μM).

结论:

*Streptomyces coeruleorubidus* NRRL B-2569是一种有效的生物催化剂,用于调节黄.
与原始化合物相比,生物合成衍生物显示出优越的水溶性和增强的生物活性.
这种方法为开发改进的基于黄类药物的治疗方法提供了一个有希望的策略.

关键词:

2′-氧黄一个这是一种抗癌药物.抗氧化剂是一种抗氧化剂.葡萄糖的化氧化液的氧化方式

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