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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Membrane protein structure and dynamics probed by MicroED.

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pH-mediated activation of the lysosomal arginine sensor SLC38A9.

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Direct from the seed: an atomic resolution protein structure by ab initio MicroED.

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Rapid Structural Analysis of Natural Products Using MicroED.

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β-barrels from short macrocyclic peptides.

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Discovery of Oxyacanthine Dihydrochloride Monohydrate Polymorphs from Obfuscated Samples by Microcrystal Electron Diffraction.

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High-throughput measurements of protein domain functions using magnetic separation.

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Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

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Photocatalytic low-temperature defluorination of PTFE.

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Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

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相关实验视频

Updated: Jun 4, 2025

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
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A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

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综合的微晶电子衍射样本准备用于冷-EMEM.

William J Nicolas1, Cody Gillman2,3, Sara J Weaver2

  • 1Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA, USA.

Nature protocols
|December 20, 2024
PubMed
概括
此摘要是机器生成的。

本指南详细介绍了微晶电子衍射 (MicroED) 用于各种分子和蛋白质的样品制备. 它为使用这种先进的冷电子显微镜 (cryo-EM) 技术的研究人员提供了优化策略.

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Optimizing Sample Preparation for Cryogenic Electron Microscopy
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Optimizing Sample Preparation for Cryogenic Electron Microscopy

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Miniaturized Sample Preparation for Transmission Electron Microscopy
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Miniaturized Sample Preparation for Transmission Electron Microscopy

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相关实验视频

Last Updated: Jun 4, 2025

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A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

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Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

269
Miniaturized Sample Preparation for Transmission Electron Microscopy
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科学领域:

  • 结构生物学是结构生物学.
  • 生物物理学的生物物理.
  • 材料科学是一种材料科学.

背景情况:

  • 微晶电子衍射 (MicroED) 是一种强大的冷电子显微镜 (cryo-EM) 技术,用于确定晶体结构.
  • 它适用于各种样品,包括小分子,可溶性蛋白质和膜蛋白质.
  • 在MicroED样本准备,数据采集和处理方面的专业知识并不总是容易获得的.

研究的目的:

  • 提供关于MicroED样品准备的全面指南.
  • 针对各种样本类别的常用方法.
  • 为 MicroED 样本准备提供指令和优化策略.

主要方法:

  • 详细介绍了MicroED样品准备的详细协议.
  • 方法涵盖室温固态小分子,可溶性蛋白质和膜蛋白质晶体.
  • 针对独特的晶体生长和制备条件,包括了优化策略.

主要成果:

  • 该指南为MicroED.的新用户提供了实用指南.
  • 它为优化各种晶体材料的样本准备提供了一个框架.
  • 该协议适用于生物化学,晶体学和冷EM现有专业知识的研究人员.

结论:

  • 本资源旨在提高对MicroED技术的可访问性.
  • 有效的样本准备对于成功确定MicroED结构至关重要.
  • 该指南使研究人员能够克服MicroED样本准备方面的挑战.