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柏柏林促进了原始卵泡的激活,并增加了老年小鼠的排卵卵卵子数量
Shuang Liu1, Weiyong Wang1, Huiyu Liu1
1The Innovation Centre of Ministry of Education for Development and Diseases, School of Medicine, South China University of Technology, Guangzhou, 510006, China.
Molecular medicine (Cambridge, Mass.)
|December 20, 2024
概括
柏柏林 (BBR) 通过PI3K/Akt通路促进小鼠和人类的原始毛囊激活. 这种天然化合物还可以提高老年小鼠的卵细胞数量,这表明POI和老年妇女的潜在生育益处.
科学领域:
- 生殖生物学 生殖生物学
- 药理学 药理学是指药理学的学科.
- 卵巢生理学 卵巢生理学
背景情况:
- 原始卵泡激活对女性生育至关重要,特别是在晚年孕产妇和早产卵巢衰竭 (POI) 的情况下.
- 活体中激活休眠原始毛囊的当前治疗选择有限,缺乏已证实的安全性.
- 柏柏林 (BBR) 是一种天然化合物,具有多种药理作用,但其在原始卵泡激活中的作用以前是未知的.
研究的目的:
- 研究柏柏林 (BBR) 对小鼠和人类卵巢组织原始卵泡激活的影响.
- 阐明BBR影响毛囊发育的分子途径.
- 评估BBR在老年雌性小鼠中提高卵细胞产量的潜力.
主要方法:
- 新生儿小鼠卵巢在体外培养并用BBR治疗;BBR也通过腹膜内注射进行.
- 用BBR培养人类卵巢碎片,以评估其影响.
- 年龄较大的小鼠接受了胃内BBR,以评估其在排卵后对卵细胞数量的影响.
- 分析PI3K/Akt通路时使用西方斑点和光强度测量,并使用LY294002 (PI3K抑制剂) 来确认该通路的参与.
主要成果:
- 在新生小鼠卵巢中,BBR显著增加了生长的毛囊数量和酸化Akt (p-Akt) 的水平,无论是体外还是体内.
- BBR治疗导致原始毛囊内的卵细胞中的p-Akt水平升高.
- BBR对卵泡生长和p-Akt的积极影响被PI3K抑制剂LY294002.2逆转.
- 在老年小鼠中,BBR的口服增加了排卵卵细胞的数量.
- 在培养的人类卵巢碎片中,BBR提高了生长卵泡的比例,并增加了p-Akt水平.
结论:
- 柏柏林 (BBR) 有效地促进了老鼠和人类卵巢的原始卵泡激活.
- 这种机制涉及到卵细胞内PI3K/Akt信号通路的激活.
- 在老年小鼠中,BBR的使用改善了卵细胞的产量,这表明在POI和高级生殖年龄的妇女中增强生育能力的潜在治疗应用.
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