3D培养通过UQCRC2-介导的线粒体氧化酸化抑制SCAPs的复制性衰老
Yijia Shi1,2,3, Tong Xiao1,2,3, Yingying Weng1,2,3
1Department of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Journal of translational medicine
|December 20, 2024
概括
来自上的三维干细胞培养 (SCAPs) 通过维护线粒体平衡来延缓衰老. 这涉及调节UQCRC2以支持氧化酸化和减少活性氧物种,帮助组织工程应用.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 牙上 (SCAPs) 的干细胞对于牙根的发育和牙脉的再生至关重要.
- 在实验室中SCAP的扩张导致复制性衰老,限制了它们的临床应用.
- 开发减轻SCAP衰老的策略对于组织工程至关重要.
研究的目的:
- 研究三维 (3D) 甲基纤维素培养环境对SCAP衰老的影响.
- 阐明3D培养介导衰老延迟的潜在机制,特别是线粒体功能和UQCRC2参与.
- 为在体外培养过程中保存SCAP功能提供一种新的方法.
主要方法:
- 在3D甲基纤维素环境中培养SCAP.
- 对细胞衰老进行了评估.
- 分析了线粒体功能,包括氧化酸化和反应性氧物种的产生.
- 用转录组高通量测序来确定关键的监管因素.
- 进行了UQCRC2基因淘汰,以验证其作用.
主要成果:
- 与传统的2D培养相比,3D培养显著延迟了SCAP衰老.
- 3D培养调节了线粒体功能和氧化酸化.
- 转录组分析发现UQCRC2是潜在的关键调节器.
- 在3D培养中除UQCRC2通过增加ROS,减少线粒体膜潜力和减少氧气消耗来加速衰老.
- 维持UQCRC2-介导的线粒体平衡与衰老缓解有关.
结论:
- 使用甲基纤维素的3D培养有效地减轻了SCAP衰老.
- 该机制涉及维护UQCRC2-介导的线粒体平衡,并支持氧化酸化.
- 这种方法为提高SCAP在再生医学和临床翻译中的实用性提供了一个有希望的策略.
更多相关视频
10:39A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
Published on: September 17, 2020
6.2K
08:15Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
Published on: February 3, 2022
3.0K
相关概念视频
Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
Mitochondria
10.7K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
10.7K
Replication in Eukaryotes
13.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.1K
Electron Transport Chain: Complex I and II
11.3K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.3K
Mitochondrial Membranes
8.3K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
8.3K
