在Acinetobacter baumannii MCC 311414中,铜诱导抗生素耐药性的增加
Ravi Chauhan1, Hardi Patel1, Bhavna Bhardwaj1
1Microbiology Lab, School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, 382030, India.
概括
接触铜显著增加了Acinetobacter baumannii的抗生素耐药性,这是一个常见的医院病原体. 这种环境压力增强了细菌的生存,并促进了抗菌素耐药性的传播.
科学领域:
- 环境微生物学 环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 细菌病原体的产生
背景情况:
- 宝曼尼菌 (Acinetobacter baumannii) 是一种具有越来越强抗生素耐药性的临床病原体.
- 环境污染物,如铜,可能有助于传播抗菌素耐药性.
- 了解环境压力因素对A. baumannii的影响对于公众健康至关重要.
研究的目的:
- 调查铜应激对A. baumannii.抗生素耐药性的影响.
- 分析铜压力A. baumannii. 的表型和基因型变化.
- 探索环境因素在增强抗菌素耐药性的作用.
主要方法:
- A. baumannii MCC 3114 暴露于铜应力.
- 确定抗生素耐药性水平对胆固醇素,西普罗素和利沃素.
- 扫描电子显微镜 (SEM) 用于生物膜和细胞外聚合物质 (EPS) 分析.
- 福里埃转换红外光谱 (FTIR) 检测EPS结构变化.
- 评估反应性氧物种 (ROS) 耐受性,排泄活动和膜透性.
- 基因表达的分子分析 (排泄,氧化应激,整体和抗生素耐药性基因).
主要成果:
- 铜应激诱导耐药性显著增加:对胆固醇素是8倍,对西普罗素是44倍,对利沃素是22倍.
- 适应的A. baumannii表现出增强的生物膜形成,具有更密集的EPS,改变的结构性质和增加的ROS耐受性.
- 生理变化包括排泄活动增加和膜透率降低.
- 分子分析揭示了与排泄,氧化应激,整体和抗生素耐药性相关的基因的上调表达.
结论:
- 暴露于铜的作用是一种适应性压力,导致A. baumannii.增强抗生素耐药性.
- 医院环境和水库中的环境铜可以推动基因型和表型变化,加剧抗菌素耐药性危机.
- 这项研究强调了环境污染物与抗生素耐药性病原体的进化之间的联系.
相关概念视频
01:30Development of Antibiotic Resistance
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
00:57Antibiotic Selection
52.3K
Overview
52.3K
01:33Bacterial Transformation
55.1K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
55.1K