细菌纯素代谢通过DAF-16调节C. elegans的发育和应激耐受性
Min Feng1, Baizhen Gao1, L Rene Garcia2
1Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.
The FEBS journal
|December 21, 2024
概括
来自细菌的饮食纯素代谢中间体可以影响动物的发育. 具体来说,Escherichia coli purE突变延迟了Caenorhabditis elegans的发展,激活了DAF-16通路并增强了抗压能力.
科学领域:
- 代谢调节 代谢调节 代谢调节
- 发育生物学是发展生物学.
- 微生物与宿主相互作用
背景情况:
- 精氨酸代谢对所有生命形式至关重要,但其在动物发育中的作用在受到食细菌中间体的影响时尚不清楚.
- 凯诺哈比蒂斯 (Caenorhabditis elegans) 作为一种模型甲基动物,用于研究这些宿主-微生物代谢相互作用.
研究的目的:
- 研究扰乱细菌纯素生物合成如何影响动物发育,并确定潜在的机制.
- 探索FOXO转录因子DAF-16在调解这些效应中的作用.
主要方法:
- 对于对C. elegans的发育影响,对Escherichia coli突变库 (Keio集合) 的高通量查.
- 代谢物补充测试以确定特定的抑制化合物.
- 用RNA测序和表型分析分析分子和生理反应.
主要成果:
- 确定了34种大肠杆菌突变,包括purE,以剂量依赖的方式延迟了C. elegans的发展.
- 发现细菌代谢物5-aminoimidazole-4-carboxamide ribotide (AICAR) 抑制了虫的生长.
- 纯E突变导致C. elegans的核DAF-16积累增加,寿命延长,耐热性和病原体耐药性增加.
结论:
- 细菌纯素生物合成中间体可以作为信号分子,影响宿主发育和防御.
- 该DAF-16通路对于调解由大肠杆菌纯E突变引起的发育延迟和应激反应至关重要.
- 这项研究突出了一个新的跨王国通信通道,影响宿主生理学.
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