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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jun 4, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

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使用Open-ST进行高分辨率3D空间转录组的协议.

Marie Schott1, Daniel León-Periñán1, Elena Splendiani2

  • 1Laboratory for Systems Biology of Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Max-Delbrück-Centrum for Molecular Medicine in the Helmholtz Association (MDC), Hannoversche Str. 28, 10115 Berlin, Germany.

STAR protocols
|December 21, 2024
PubMed
概括
此摘要是机器生成的。

本研究介绍了Open-ST,这是一个用于2D和3D组织样本的高分辨率空间转录学 (ST) 的新协议. 开放ST使得健康和疾病研究的详细分子绘图.

关键词:
在RNA-seqqq.生物信息学是一种生物信息学.癌症 癌症 癌症 癌症 癌症显微镜 显微镜是指使用显微镜.系统生物学 系统生物学

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Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

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相关实验视频

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Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 空间转录学 (ST) 对于剖析生物组织中的分子机制至关重要.
  • 了解空间基因表达模式是推动健康和疾病研究的关键.

研究的目的:

  • 为2D和3D空间转录学提供一个高效和高分辨率的协议.
  • 介绍Open-ST,一种用于生成组织分子图的新型计算工作流.

主要方法:

  • 将Illumina流动单元重新定位为空间条形码的捕获区域.
  • 从染色的冷切片准备空间转录图书馆.
  • 详细的计算工作流程用于生成与组织学一致的2D/3D分子图.

主要成果:

  • 为高效和高分辨率的空间转录学开发开放式ST协议.
  • 创建"虚拟组织块",将分子数据与空间信息集成在一起.
  • 证明Open-ST对包括临床样本在内的各种组织类型的适用性.

结论:

  • 开放ST提供了一个强大的方法,用于各种组织类型的高分辨率空间转录学.
  • 该协议有助于在它们的空间上下文中探索分子路径.
  • 这种方法提高了对健康和疾病分子机制的理解.