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Updated: Jun 4, 2025

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

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增强的电子转移,以提高去除效率和在低温下减少N₂O

Bangrui Lan¹, Chunlei Liu¹, Shanyun Wang¹

¹Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

|December 21, 2024

概括

低温阻碍了生物过程. 在建造的湿地中,向微生物电解细胞施加微电流可以促进电子转移,增强去除,减少在寒冷条件下的氧化排放.

科学领域:

环境微生物学环境微生物学
生物地质化学生物地质化学
废水处理废水处理

背景情况:

低温阻碍了微生物活动,减缓了必要的生态化学循环,导致了污染.
在寒冷的环境中,生物除过程的效率显著下降.

研究的目的:

调查微电流刺激在提高微生物在低温下的电子转移中的有效性.
评估微流对冬季建筑湿地中污染物清除和氧化 (N2O) 排放的影响.

主要方法:

使用太阳能驱动的微生物电解电池与建造的湿地系统相结合.
通过酶复合活性测定和基因转录 (RT-qPCR) 测量微生物活性.
量化去除,N2O生产和微生物社区转移使用同位素标记和高通量测序.

主要成果:

微电流 (18.9 ± 5.5 μA) 显著增加了电子运输复合体 (II,III,IV) 的活性和关键循环基因 (amoA,nirS/K) 的转录.
-N和-N的去除效率分别提高了12%和10%,而N2O排放量减少了44%.
刺激导致特定的化和脱细菌的相对丰度显著增加,特别是Nitrospira (156%的增加).

结论:

微电流刺激是一种可行的策略,可以增强微生物活动和在寒冷条件下去除.
这种方法为减轻污染和减少冬季建造湿地温室气体排放提供了一种新方法.
这些发现突出了在低于最佳温度下运行的生物系统的电生化增强潜力.

关键词:

建筑湿地是建造的湿地.电子转移是指电子的转移.低温低温低温的温度是什么减少排放的N(2) O减少排放移除的方法移除的方法

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