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通过调节GPX4和SEPP1促进神经发育,这种调节发生在iPSC衍生的神经模型中

Zhenzhu Dai¹, Yanzi Yu¹, Ruhai Chen¹

¹State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs, Key Laboratory of CNS Regeneration (Ministry of Education), Guangdong Key Laboratory of Non-Human Primate Research, GHM Institute of CNS Regeneration, Department of Chemistry, Jinan University, Guangzhou, 510632, China.

|December 21, 2024

概括

纳米粒子 (SeNPs@LNT) 通过促进细胞增殖和分化来增强神经发育. 这项研究揭示了SeNPs@LNT.

科学领域:

神经科学是一个神经科学.
生物化学生物化学
发展生物学发展生物学

背景情况:

含有单类固醇的蛋白对神经发育至关重要,但它们的确切作用仍然难以捉摸.
了解神经元发育各个阶段的蛋白功能对于解决神经发育障碍至关重要.

研究的目的:

通过诱导多能干干细胞衍生神经模型 (iPSC-iNeuron) 研究纳米颗粒 (SeNPs@LNT) 对神经发育的影响.
阐明神经发育过程中SeNPs@LNT调节蛋白表达和信号通路的机制.

主要方法:

建立了一个iPSC-iNeuron模型来研究神经发育.
在不同发育阶段给予SeNPs@LNT,以评估其影响.
使用基因表达分析 (例如GPX4,SEPP1) 和信号通路分析 (PI3K/Akt/Nrf2).
分析了来自自闭症谱系障碍 (ASD) 患者的单细胞RNA-seq数据.

主要成果:

SeNPs@LNT通过谷氨过氧化酶4 (GPX4) 的上调加速了神经前体细胞 (NPC) 的增殖.
SeNPs@LNT通过增加蛋白P (SEPP1) 来促进神经元分化.
SeNPs@LNT激活了PI3K/Akt/Nrf2通路,调节蛋白质并影响神经发育.
在ASD患者神经元中较低的GPX4表达与线粒体功能障碍和异常蛋白合成相关.

结论:

通过调节蛋白质,SeNPs@LNT对早期神经发育具有有益影响.
这些发现表明SeNPs@LNT在神经发育障碍中的潜在治疗作用.
异常的蛋白合成和线粒体功能障碍可能会导致ASD的发病.

关键词:

神经发育神经发育在SeNPs@LNT上发送.蛋白质是一种蛋白蛋白质.在 iPSC-iNeuron 模型中.

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