整个生命周期中蛋白质丰富性的DNA甲基化模型
Scott Waterfield1,2,3,4, Paul Yousefi5,6, Matt Suderman5,6
1MRC Integrative Epidemiology Unit, University of Bristol, Bristol, UK. scott.waterfield@bristol.ac.uk.
Clinical epigenetics
|December 21, 2024
概括
称为表皮质的DNA甲基化 (DNAm) 模型可以预测蛋白质水平. 这些模型捕捉了蛋白质丰富度的非遗传变异,在成年人中表现比儿童更好.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 蛋白质组学是指蛋白质组学.
- 生活历程 流行病学 流行病学
背景情况:
- 蛋白质丰度的DNA甲基化 (DNAm) 模型对于了解暴露,表型和疾病风险是有价值的.
- 研究DNAm能够捕捉整个生命周期中蛋白质丰度的非遗传变异的能力至关重要.
研究的目的:
- 评估现有的DNAm模型 (表皮) 的性能,以预测蛋白质丰富度.
- 评估DNAm在不同生命阶段捕获非遗传蛋白质变异的能力.
- 开发和验证新的特定年龄段的表皮记忆.
主要方法:
- 利用了来自阿文长度研究父母和儿童 (ALSPAC) 队列的外周血液样本,包括母亲和后代 (7-24岁).
- 评估了14个已发表的DNAm表皮质,并训练了新的特定年龄表皮质.
- 评估了带有和没有遗传调整的表皮蛋白关联,并评估了纵向蛋白质和表皮蛋白稳定性.
主要成果:
- 在14种表皮质中,10种表皮质与中年人群的蛋白质丰富性有关,24岁时有9种表皮质,9岁时没有表皮质.
- 八个表皮解释了成年后的基因型之外的变异.
- DNAm模拟了9岁 (22 种蛋白质),24岁 (31 种蛋白质) 和中年 (23 种蛋白质) 的蛋白质丰度,不同程度的非遗传贡献.
结论:
- 与儿童相比,Episcores在老年人中表现得更好.
- 在所有评估年龄段,几种表皮细胞始终捕获了蛋白质丰度的非遗传变异.
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