法医代谢学:追踪化物诱导的代谢变化在死亡事故中
Wilmar Alexander Ariza-Garcia1, Daniel Pardo-Rodriguez2, Gloria E Guerrero Alvarez3
1Universidad Tecnológica de Pereira, Risaralda, CPA 660003, Pereira, Colombia; Instituto Nacional de Medicina Legal y Ciencias Forenses (INML y CF), Pereira, Risaralda 660001, Colombia.
Forensic science international
|December 22, 2024
概括
检测化物中毒是困难的,因为它的挥发性. 这项研究确定了关键的代谢变化和潜在的生物标志物,如酸和3甲酸,以改善对化物暴露的法医诊断.
科学领域:
- 法医毒理学 法医毒理学
- 代谢学 代谢学 代谢学
- 生物化学 生物化学
背景情况:
- 化物暴露的检测在法医中至关重要,但由于化物的挥发性和样本转换而具有挑战性.
- 替代代物代谢物标记物可以提高检测准确度,并扩大化物中毒病例的法医窗口.
研究的目的:
- 为了调查在暴露于化物后的法医病例中的代谢变化.
- 通过多平台的代谢学和脂质学方法识别化物中毒的潜在生物标志物.
主要方法:
- 多平台代谢学和脂质学分析使用液体和气体染色学与高分辨率质谱学相结合.
- 对群体歧视进行正交部分最小方程差异分析 (OPLS-DA).
- 接收器运行特征 (ROC) 曲线分析用于预测性价值评估.
主要成果:
- 在暴露于化物和对照组之间实现了明确的歧视.
- 确定了92种改变的代谢物,其中糖脂,糖脂,脂肪酸和脂的显著变化.
- 化物中毒破坏了关键的新陈代谢途径,包括β-氧化,氨基酸代谢和氨基稳态.
结论:
- 改变的代谢概况提供了关于化物中毒机制的见解.
- 酸盐和3酸酸等代谢物显示出作为致命的化物中毒的诊断生物标志物具有前途.
- 需要在不同的队列中进一步验证,以建立可靠的法医诊断小组.
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