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使用纸张传感器在食品中快速和灵敏地定量蛋白溶酶

Jialin Hu¹, Aihong Wu¹, Liqiang Liu¹

¹International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

|December 22, 2024

概括

一种新的黄金免疫染色学测定 (GICA) 准确检测食物中的蛋白溶酶 (HEWL). 这种快速方法通过量化常见的蛋过敏原HEWL来确保食品安全,防止过敏反应.

科学领域:

食品科学食品科学食品科学
检测过敏原的检测方法
免疫学检测免疫学检测

背景情况:

蛋白溶酶 (HEWL) 广泛用于食品保存.
是重要的蛋过敏原,对过敏消费者构成风险.
现有的检测方法可能会受到食品矩阵的影响.

研究的目的:

为在食品中检测HEWL开发一种特定且对矩阵耐受的测定方法.
量化各种食品中的HEWL水平.
通过识别潜在的过敏原污染来确保消费者的安全.

主要方法:

开发一种特定于HEWL.L.的单克隆抗体 (mAb).
针对mAb相互作用分析的三明治酶相关免疫吸收试验 (ELISA) 的优化.
使用mAb和合金制造黄金免疫染色学测试 (GICA) 条带.
使用HEWL负的食物矩阵 (牛奶,奶酪,葡萄酒) 建立标准曲线,以减轻矩阵效应.

主要成果:

该GICA方法证明了HEWL检测的高特异性和灵敏性.
牛奶 (21.12 ng/mL),奶酪 (0.89 μg/kg) 和葡萄酒 (1.61 ng/mL) 的检测极限已经确定.
恢复率在99.16%至102.25%之间,相当于高性能液体染色学 (HPLC) 的结果.

结论:

成功开发了一种快速,灵敏和特定的GICA方法,用于牛奶,奶酪和葡萄酒中的HEWL合格和定量.
该分析对矩阵效应具有耐受性,因此适用于复杂的食品样本.
这种方法有助于改善食品安全和食品工业中的过敏原管理.

关键词:

过敏原是一种过敏原.黄金免疫染色学测定方法蛋白素酶是一种蛋白素.单克隆抗体是一种单克隆抗体.

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