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骨肌纤维中的Mll4维护肌肉干细胞

Yea-Eun Kim¹, Sang-Hyeon Hann¹, Young-Woo Jo¹

¹School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.

Skeletal muscle

|December 22, 2024

概括

混合系白血病4 (Mll4) 是维持年轻和成人肌肉肌肉干细胞 (MuSCs) 的必要条件. 它的缺失导致MuSCs过早分化,导致肌肉消耗.

科学领域:

肌肉生物学肌肉生物学
干细胞研究的研究.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

肌肉干细胞 (MuSCs) 对于肌肉生长和修复至关重要,经历关键状态过渡.
表观遗传修饰会影响 MuSC 利基和细胞状态.
混合血统白血病4 (Mll4),一种组氨基甲基转移酶,在骨肌肉中高度表达,表明其具有调节作用.

研究的目的:

调查肌纤维表达的Mll4在维护MuSCs中的作用.
为了确定Mll4是否调节MuSC状态转换.

主要方法:

通过将MCK^Cre/+或HSA^MerCreMer/+小鼠与Mll4^f/f小鼠交叉生成肌纤维特定的Mll4删除小鼠.
在不同年龄 (青春期和成人) 的小鼠中使用了构成性和诱导性Mll4剥离模型.

主要成果:

发育中的肌肉中Mll4的缺失导致循环MuSCs的提升过早分化,导致MuSC耗尽.
在成年肌肉中,Mll4剥离导致MuSCs失去静止和分化,也导致枯竭.
在肌纤维中,Mll4剥离与减少Notch配体 (Jag1,Dll1,Jag2,Dll4) 的表达相关.

结论:

肌纤维表达的Mll4对于维持青春期和成人骨肌肉中的MuSCs至关重要.
Mll4很可能通过调节肌纤维中的Notch配体表达来维持MuSCs.
Mll4 作为 MuSCs 的主调节者,影响发育和成人的肌体形成,独立于性别.

关键词:

运动运动运动.肌肉干细胞是肌肉的干细胞.骨髓性/淋巴细胞性或混合血统白血病4 (Mll4)肌纤维纤维是一种纤维纤维.口信号传输是没有的.骨架肌肉是一个骨肌肉.

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