在LC-ESI-MS/MS中由冠状病毒排放人工物诱导的芳香代谢物的幽灵峰值
Yayoi Hongo1, Daisuke Fukuyama2, Lee Chuin Chen3
1Okinawa Institute of Science and Technology (OIST), Okinawa, Japan.
Journal of mass spectrometry : JMS
|December 23, 2024
概括
在液体染色学-电子喷雾电离-并联质谱学 (LC-ESI-MS/MS) 中的源氧化可以为多巴胺等代谢物产生人工物"幽灵峰". 了解这种胺氧化是早期动物研究中准确代谢的关键.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 进化生物学 进化生物学
背景情况:
- 液体染色学-电子喷射电离-并联质谱学 (LC-ESI-MS/MS) 对于代谢物识别至关重要,特别是在像Cnidaria这样的早期分离动物中.
- 基础甲基动物的新陈代谢尚不清楚,这使得识别它们独特的代谢产物变得更加复杂.
- 意想不到的工件峰值,例如多巴胺.
研究的目的:
- 为了调查在LC-ESI-MS/MS分析中观察到的"幽灵峰值"的人工制品的原因,该分析对 cnidarian Nematostella vectensis.中的多巴胺进行了分析.
- 阐明LC-ESI-MS/MS中源氧化器件的机制.
- 在复杂的生物样本中建立准确的代谢物识别方法.
主要方法:
- 液体染色学-电子喷雾电离-并联质谱学 (LC-ESI-MS/MS) 对Nematostella vectensis的分析.
- 验证实验以确定文物峰值的来源.
- 研究源内氧化机制,包括冠状排放和使用18O标记水的氧气源的作用.
主要成果:
- 发现了一种具有与多巴胺相同的质量电荷比率 (m/z) 和MS/MS频谱的"幽灵峰".
- 铁氨酸的源氧化被证实是多巴胺"幽灵峰"的原因.
- 冠状病毒排放被确定为源氧化的主要因素,有证据表明有多个氧气来源.
结论:
- 在源氧化工件可以在LC-ESI-MS/MS分析中模仿内源代谢物,导致误识.
- 充分的染色分离对于将人工物与真正的代谢物区分开来至关重要.
- 准确的代谢概况,特别是在研究不足的生物体中,需要仔细考虑潜在的源氧化产物.
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